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CANADIAN HEAD OFFICE
1232 Kingston Road, Toronto, Ontario, Canada, M1N 1P3

US LABORATORY and RESEARCH CENTER

2500 COLLECTION CENTERS ACROSS NORTH AMERICA TO SERVE EVERY STATE AND PROVINCE
FREE professional and confidential consultation: Call 1-877-842-4827 or 416-691-4167

Call Today for a FREE consultation: 1-877-842-4827 or 416-691-4167
1232 KINGSTON RD.,TORONTO, ONTARIO, CANADA, M1N 1P3
COLLECTION CENTERS ACROSS NORTH AMERICA TO SERVE EVERY STATE AND PROVINCE

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All Tests Can Be Done For Legal Purposes.
Every Viaguard Test is Available Internationally.
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Corticosterone (C21H30O, Kendall's Compound B) is a glucocorticoid secreted by the cortex of the adrenal gland in response to the stimulation by adrenocorticotropic hormone. Corticosterone is a major indicator of stress in non-human mammals. Glucocorticoids, such as corticosterone, guide fundamental processes associated with converting sugar, fat, and protein stores to useable energy; inhibiting swelling and inflammation; and suppressing immune responses following a stress event.

Corticosterone regulates energy metabolism and the response to stress, aimed at maintaining homeostasis by controlling the stress response system sensitivity and by facilitation of homeostasis after a stress-induced disturbance action linked to G proteins (8). Corticosterone has a pleiotropic action and induces multiple changes in peripheral responses that indirectly influence brain function. This has been shown for responses of the immune system and the cardiovascular system and after corticosterone-induced changes in carbohydrate and mineral metabolism.

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Cortisol & the Burn Out Syndrome

Recent studies show that cortisol levels are an indicator of 'burn out levels'. Burnout reduces cortisol levels below the normal range, meaning people do not react adequately to testing situations. Further, people feeling burnout are often diagnosed as depressed and given anti-depressants which further lower the cortisol level. A saliva test for cortisol gives a short term window, but the Viaguard hair test provides a meaningful ninety day perspective on cortisol levels.

Quantitative Measurement of Your Stress Level

Stress is caused by a variety of external factors and is also self generated. The symptoms are varied and include a variety of cognitive, emotional, physical and behavioural symptoms.

Stress is increasingly recognized as a major factor in mental and physical well-being. There are an increasing number and variety of practitioners who have been assisting clients and patients by employing their training and years of experience to deal with the multiplex of stress problems.

Research studies show that everyday stress reshapes the brain-nerve cells change their morphology. The number of connections with other cells and the way they communicate with other neurons is affected. In most cases, these responses are adaptive and beneficial-they help the brain cope with stress and shape adequate behavioural reaction. However, upon severe stress things can get out of control, the brain buffering capacity is exhausted and the nerve cells in the hippocampus – an area of the brain responsible for learning and memory – start to withdraw their processes, don’t effectively communicate with other cells and show signs of disease.

The critical importance of stress and cognitive development in adolescence

Chronic repetitive stress has a more powerful effect on the brain during adolescence than in adfulthood.Research indicates that stress in adolescence causes a molecular loss of glutamate receptors. a key factor in mental illness. A loss of glutamate receptotrs in the prefrontal cortex, the area of the brain that controls memory, and decision making can lead to later significant cognitive impairment. While there have been many behavioural studies about stress, understanding stress at the molecukar level creates new treatment possibilities. The cortisol level , in hair, is the one measurable indicator of stress level.

Now, the quantitative measurement of stress is possible. Years of research at leading institutions in Canada, the US, and other countries have determined that the quantitative analysis of cortisol deposited over a 90- to 100-day period in the cortex of the hair shaft, is an extremely accurate, quantitative reflection of chronic stress levels during that period.

The research was originally intended to validate the relationship between chronic stress levels over that period and an individual’s vulnerability to acute myocardial infarction (heart attack) and has proved to be a valuable tool in that regard.

Perhaps the most significant role of quantitative stress level determination will be, as a starting point, in an individuals understanding of what their chronic stress level is. This is an important guide to any subsequent treatment. Viaguard laboratories, a leading Canadian DNA and drug testing facility, is now marketing a 90-day hair cortisol test that determines your stress level.

Hair sample collection kits are supplied at no cost and the laboratory analysis fee of $149.00 is remitted when the hair sample is submitted for analysis. The kit contains complete, easy-to-use instructions for collecting the remitting the sample.

Cortisol levels in tested individuals range between about 100 ng/g and 1000ng/g and when the analysis is completed, the report and conclusions are documented. For additional information, contact Viaguard at 416-691-4167 (toll free 1-866-842-4827)

Heart disease, mainly heart attacks are the number one cause of death in adults. Worldwide, 29% of all adult deaths are linked to heart disease and the majority of these are sudden deaths that occur outside a hospital.


While heart attacks are caused by a variety of factors including stress, lifestyle, genetics, physiology, etc., a study released in September 2010 shows the very important correlation between the risk of a heart attack and the stress levels as reflected in the level of cortisol deposited and stored in a person’s hair. The cortex of the hair shaft usually holds up to six months of deposited cortisol, providing a measureable record of stress levels. Stress levels have been identified as a significant indicator of the potential for heart attacks. The study was published in the International Journal on the Biology of Stress. The research was carried out at leading universities and institutes in Canada, the U.S.A., and internationally and those studies have confirmed the direct relationship between cortisol levels and predisposition to a heart attack. The studies were widely publicized in the media because of their importance.


The ability of cortisol to be deposited in hair is related to its chemical nature. The cell membrane complex that makes up the interior cortex of the growing hair shaft consists of proteins and a protein-lipid complex originating from previous cell membranes. Cortisol easily enters the growing hair shaft and diffuses into the interior matrix cells where it is stored.


Cortisol is released by the adrenal gland in reaction to stress and ultimately deposited in the cortex of the hair shaft by diffusion from the capillary system abutting hair follicles.


Stress has been identified as a major factor in a variety of medical conditions but for the first time these extensive studies have indicated a significant linkage between acute myocardial infarction (heart attack) and cumulative stress levels as evidenced by cortisol deposition in the hair.


The HPA Axis and Stress


The hypothalamus/pituitary-adrenal glands (HPA) have a complex and interconnected influence which control reactions to stress. There relationship results in the production of corticotropic releasing hormones (CRH) and vasopressin. These agents stimulate production of ACTH (Adrenocorticotropin hormone) and ACTH in turn stimulates the biosynthesis of cortisol. Cortisol is the hormone that initiates reactions to stress.


While cortisol is carried in the bloodstream to multiple receptor sites, it is also deposited in hair where it is retained in the cortex of the hair shaft. A sample of hair provides a 90 day record of cortisol production which is statistically linked to stress levels. These levels are a valuable barometer of the potential for an acute myocardial infarction (AMI) commonly known as a heart attack.


Extensive studies carried out at many research institutions over an extended period of time have indicated that cortisol levels in hair are a valid predictor of potentially acute myocardial infarction. The Viaguard analysis of your hair sample will determine the cortisol level and indicate the relative risk based on this level.


Heart attacks are of course a complex phenomena with a wide variety of causative factors, but determining the cortisol level is a significant breakthrough. If the results indicate an at-risk level of cortisol, you will of course be advised to discuss the situation with your physician.


What does your stress level as measured by the Viaguard Cortisol hair analysis mean?


In addition to its relation to heart attack vulnerability, chronic stress is a catalyst which accelerates the spread of a wide variety of disease conditions including cancer. Research indicates that stress biologically reprogrammed certain body defence mechanisms, diminishing their protective ability. A 2010 study published in the Journal Cancer Research indicates that high chronic stress levels cause cancer cells to migrate from a primary tumour and attack various organs. Stress does not directly cause disease, but is a facilitator of disease conditions.


Knowing your cronic stress level is an important guide which clinicians may use to recommend changes in lifestyle and medication.


Elevated levels of cortisol provide an important, early warning sign of a potentially acute event. If levels are found to be elevated, the prudent course would be consultation with your physician. The Viaguard analysis of your hair cortisol levels will clearly indicate the relative level of risk, but it is important to remember that stress level, as reflected by cortisol, is one of a multiplex of factors that relate to heart attacks, but apparently one with great significance.


The Viaguard sample hair collection kit will allow you to collect a 1.5” or 2” (or longer) sample of scalp hair and submit it to our laboratory for analysis of your 90-day cumulative cortisol level as it relates to stress and our recommendations.


The cost of the test is $149.


For further information, please call 416-691-4167 or email us at info@accu-metrics.com.

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